PEGylation is a chemical process to covalently attach PEG polymers to a substrate including molecules, macromolecules, particles, surfaces etc. Based on reactive sites in the substrate entities, PEGylation requires different functionalities on PEG reagents. The list below gives several examples of commonly used PEGylation chemistry based on literature data.
Amine PEGylation and N-terminal PEGylation: PEG reagents react with amine present in biological macromolecules.
PEG reagentsPEGylation
PEG-NHSThe N-hydroxysuccinimide (NHS) activated ester of PEG carboxylic acid can react with the amino group of lysine. The coupling requires only mild conditions, pH 7-9, low temperature (5-25°C) for short period of time. The formed amide bond is physiologically stable.
PEG-AldehydeReductive amination with primary amines to produce secondary amines, in the presence of reducing agents such as sodium borohydride and sodium cyanoborohydride. The pH is important for reductive amination. PEG-Aldehyde is a very good reagent for N-terminal PEGylation.
PEG EpoxideNucleophilic addition
PEG-IsothiocyanateReact with amine to produce a stable thiourea linkage.
PEG-COOHUsually the acid needs to be activated, such as NHS ester.
PEG-NPCAmine reacts with NPC functionalized PEG under proper conditions.
PEG-AcrylateMichael addition between amine and acrylate ester. It is a relatively slow reaction.
Carboxyl PEGylation: PEG reagents react with carboxylic acid in the presence of coupling agents such as DCC (N,N'-dicyclohexylcarbodiimide) and EDIC (N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide, HCl salt).
PEG reagentsPEGylation
PEG-AmineAmide formation under DCC or EDC coupling conditions
PEG-HydrazideAfter activated by EDIC at mild acidic pH, the carboxyl group of proteins readily react with PEG-hydrazide, while the amino groups present in all reagents could remain inactive in this particular conditions.
PEG-Halide/sulfonatePEG-halide (chloride, bromide, iodide, tosylate and mesylate) reacts with deprotonated carboxyl groups i.e. -COO- salt.
Thiol PEGylation: Thiol PEGylation is for free thiols of biological molecules such as cysteine.
PEG reagentsPEGylation
PEG-MaleimideMichael addition, thiols react with the C=C bond in the maleimic ring to form a physiological stable linkage. The best reaction condition is at pH 8.
PEG-OPSSDisulfide S-S bond formation, which can be reversed by reducing agents such as sodium borohydride and thioethanolamine
PEG-VinylsulfoneMichael addition, thiols react with the C=C bond to form a physiological stable linkage
PEG-ThiolOxidative disulfide S-S bond formation
PEG-HalidePEG-halide (chloride, bromide, iodide, tosylate and mesylate) reacts with free thiol. Iodoacetate or iodoacetamide is very reactive towards free thiols.
Hydroxyl PEGylation
PEG reagentsPEGylation
PEG-IsocyanateHydroxyl groups react with PEG-NCO, however special considerations are required.
PEG-NPCHydroxyl groups react with NPC to from a carbonate linkage.
PEG-SilaneSilane reacts particularly with hydroxylated surfaces.
PEG-EpoxidePEG-Epoxide reacts with hydroxyls best at pH 8.5-9.5.
Click PEGylation
PEG reagentsPEGylation
PEG-Azide
PEG-Alkyne
Azide reacts with alkyne in the prescence of copper catalyst to form a stable 1,2,3-triazole ring linkage. It is called Azide-Alkyne Huisgen Cycloaddition.
Surface PEGylation
PEG reagentsPEGylation
PEG-ThiolPEG-thiol reacts with the most common nobel metal surfaces such as gold, silver etc.
PEG-SilanePEG-Silane reacts with hydroxylated surfaces such as silica, glass, hydroxylated or acid activated nobel metal surfaces such as Pt, Pd etc.
Polymerizing PEGylation: Under radical initiators, UV illumination or high temperature, PEG crosslinkers could polymerize to form hydrogels, microparticles or crosslinked networks for drug delivery and tissue engineering
PEG reagentsPEGylation
PEG-ThiolMulti-arm PEG-thiols can crosslink itself to form disulfide bonds, which could be reversed under proper reducing conditions.
PEG-AcrylateBifunctional or multi-arm PEG-acrylate, PEG-methacrylate, or PEG-acrylamide can crosslink via stable carbon-carbon bonds.
Reversible PEGylation: Cleavable bonds could be formed using certain PEG reagents to achieve reversible PEGylation.
PEG reagentsPEGylation
PEG-HydrazidePEG-hydrazide may react with carbonyls (ketone or aldehyde) to form acylhydrazone linkage which is labile to hydrolysis under acidic conditions.
PEG-ThiolPEG-S-S-PEG disulfide bond could be cleaved with proper reducing agents.

Disclaimer: The information abovementioned serves only as a general guide for selecting PEGylation reagents. Please refer to literature publications for detailed and specific PEGylation procedures and use your own judgment. Creative PEGWorks assumes no liability for the information provided within this web page.
Copyright © 2017 Creative PEGWorks. All rights reserved.