|Catalog No. :||PSB-6112|
|Product Name :||Carbonylacrylic-PEG-Carbonylacrylic, MW 2k|
Carbonylacrylic PEG reagents are for chemoselective cysteine bioconjugation and undergo rapid thiol Michael-addition under biocompatible conditions in stoichiometric amounts. Importantly, the conjugates formed between Carbonylacrylic PEG and thiol/cysteine-containing molecules are resistant to degradation in physiological conditions. The typical PEG-maleimide-thiol reaction results in thiosuccinimide formation which is reversible, with PEG-maleimide elimination occurring slowly under biologically relevant conditions. The reversed reaction leads to the breakage of the maleimide-thiol linkage and the loss of PEG from the thiol/cysteine-containing molecules. If the maleimide-thiol chemistry does not work for your application, you may consider Carbonylacrylic PEG reagents as an improved alternative to PEG-maleimide.
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1. Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagent, Nature Communications | 7:13128 | DOI: 10.1038/ncomms13128, Text.
2. Tunable degradation of maleimide-thiol adducts in reducing environments. Bioconjug. Chem. 22, 1946â€“1953 (2011). Text.
3. Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis. Chem. Commun. (Camb.) 47, 5452â€“5454 (2011). Text.